Journal: Anesthesiology

Article Title: Platelet-derived Growth Factor Receptor-α Induces Contraction Knots and Inflammatory Pain–like Behavior in a Rat Model of Myofascial Trigger Points

doi: 10.1097/ALN.0000000000005167

Figure Lengend Snippet: Receptor tyrosine kinases (RTKs) expression profiles of myofascial trigger points (MTrPs) tissue derived from upper trapezius muscle of myofascial pain syndrome (MPS) patients and control groups. ( A ) The upregulated RTKs members were validated by microarray analysis. MPS group = 11, Con group = 7. Platelet-derived growth factor receptor-α (PDGFR-α): Con, 879 ± 83.08; 95% CI, 802.2–955.8 versus MPS, 1,060 ± 96.84; 95% CI, 994.9–1,125; units, fluorescent value; mean ± SD; P < 0.001. PDGFR-β: Con, 338.8 ± 47.96; 95% CI, 294.5–383.2 versus MPS, 308.4 ± 51.67; 95% CI, 273.7–343.1; units, fluorescent value; mean ± SD; P > 0.05. ( B ) Representative microscopic images showing morphology of muscle fibers in different groups. The morphology of MTrPs showed that annular or enlarged muscle fibers ( yellow arrows ) of different sizes with centralized nuclei in cross-sectional spaces under microscopy ( yellow arrows ). Scale bars, 20 μm. ( C ) The relationship between pain intensity and expression level of phosphorylated PDGFR-α (p-PDGFR-α) was characterized by a significant positive correlation (r = 0.711; n = 11; P < 0.05). ( D ) Results from enzyme-linked immunosorbent assay (ELISA) showed that the level of serum platelet-derived growth factor-AA (PDGF-AA) was increased. Con, 3.74 ± 0.82; 95% CI, 2.96–4.5; versus MPS, 5.97 ± 0.98; 95% CI, 5.31–6.6; units, ng/ml; mean ± SD; P < 0.001. ( E ) Results from immunohistochemistry (IHC) showed that the expression of PDGF-AA ( yellow arrows ) was upregulated at MTrPs. Scale bars, 20 μm. Con, 1.51 ± 0.33; 95% CI, 1.16–1.85; versus MPS, 10.2 ± 1.57; 95% CI, 8.55–11.85; units, integrated optical density (IOD); mean ± SD; P < 0.001. ( F ) The cross-sectional area of muscle fibers was increased in MPS group. Con, 995.2 ± 166.5; 95% CI, 902.9–1,087; versus MPS, 1,398 ± 124.2; 95% CI, 1,330–1,467; units, μm 2 ; mean ± SD; P < 0.001. ( G ) Representative fluorescence microscopic images showing expression of p-PDGFR-α ( green ) in different groups. Scale bars, 20 μm. Con, 1.00 ± 0.10; 95% CI, 0.89–1.11; versus MPS, 1.44 ± 0.20; 95% CI, 1.23–1.64; units, mean intensity; mean ± SD; P < 0.001. * P < 0.05; ** P < 0.01; *** P < 0.001. ALK, anaplastic lymphoma kinase; EphA, ephrin receptor A; EphB, ephrin receptor B; LTK, leukocyte tyrosine kinase; TRKB, tyrosine kinase receptor B; ZAP70, zeta-chain-associated protein kinase 70.

Article Snippet: Blood samples from patients with MPS were tested for platelet-derived growth factor-AA (PDGF-AA) levels using a human PDGF-AA enzyme-linked immunosorbent assay (ELISA) kit (EK1696, Bosterbio, USA).

Techniques: Expressing, Derivative Assay, Control, Microarray, Microscopy, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Fluorescence

Journal: Gene

Article Title: The role of C/EBPβ phosphorylation in modulating membrane phospholipids repairing in LPS-induced human lung/bronchial epithelial cells

doi: 10.1016/j.gene.2017.07.076

Figure Lengend Snippet: Verification of ALI model and measurement of relevant proteins. A. TNFα, IL-1β, and IL-10 in BEAS-2B cells were examined using ELISA 12 h, 24 h or 48 h after treatment of 10 mg/ml LPS. The vertical coordinate indicates the concentration of target protein, and the horizontal coordinate indicates the experiment group. B. PAF and AA contents in BEAS-2B cells were measured using ELISA 12 h, 24 h or 48 h after treatment of 10 mg/ml LPS. The vertical coordinate indicates the concentration of target protein, and the horizontal coordinate indicates the experiment group. C. CDK2, CCSP1 and PLA2 expression levels, as well as C/EBP β phosphorylation, were assessed by western blotting 12 h, 24 h or 48 h after treatment of 10 mg/ml LPS. Lower: image of target blots, upper: analysis on the optical density of three proteins, β actin serving as the reference, and for the phosphorylated protein, the total protein of itself serving as the reference. Data are means ± SD from at least three separate replicates.*, p < 0.05; **, p < 0.01. vs cell group.

Article Snippet: Total protein was extracted and quantified using the BCA method, and intracellular TNFα, IL-1β and IL-10 were measured using Human TNFα-ELISA kit, Human IL-1β-ELISA kit and Human IL-10 ELISA kit (Invitrogen).Experiments for the PAF and AA level were performed using the Human arachidonic acid (AA) ELISA Kit (MBS703581, Cayman) and Human Platelet Activating Factor (PAF) ELISA Kit (MBS701223) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Expressing, Phospho-proteomics, Western Blot

Journal: Gene

Article Title: The role of C/EBPβ phosphorylation in modulating membrane phospholipids repairing in LPS-induced human lung/bronchial epithelial cells

doi: 10.1016/j.gene.2017.07.076

Figure Lengend Snippet: Verifying the specificity of the pathway and examining membrane damage A. BEAS-2B cells were infected with the indicated lentivirus, and PLA2 expression was measured by western blotting. Upper: representative blots and lower: the optical density of the target band divided by the optical density of the β-actin band. B. BEAS-2B cells were infected with the indicated lentivirus, and treated with LPS 48 h later, and PAF and AA were measured using ELISA. Data are means ± SD from at least three separate replicates.*, p < 0.05; **, p < 0.01.

Article Snippet: Total protein was extracted and quantified using the BCA method, and intracellular TNFα, IL-1β and IL-10 were measured using Human TNFα-ELISA kit, Human IL-1β-ELISA kit and Human IL-10 ELISA kit (Invitrogen).Experiments for the PAF and AA level were performed using the Human arachidonic acid (AA) ELISA Kit (MBS703581, Cayman) and Human Platelet Activating Factor (PAF) ELISA Kit (MBS701223) according to the manufacturer's instructions.

Techniques: Membrane, Infection, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay